| Team name | Location | League | Level | Status |
|---|---|---|---|---|
| Brevard County Manatees | Viera, Florida | Florida State League | professional | defunct |
| Miami Manatees | Miami, Florida | World Hockey Association 2 | professional | defunct |
| Southwest Florida Manatees | Cape Coral, Florida | USL Premier Development League | amateur | defunct |
| Southwest Florida Manatees | Fort Myers, Florida | United Soccer Leagues-Division 2 | professional | defunct |
| State College of Florida Manatees | Bradenton, Florida | college | active |